Proposing a Model System for study of Cell-ECM Interactions in CNS Wound Healing

Abstract

Extracellular matrix (ECM) proteins play a central role in both non-neuronal wound healing and central nervous system (CNS) recovery. In both processes, the injury progresses from a plasma derived fibrin and fibronectin clot (FB-FN) to a fibronectin (FN) matrix to a scar matrix rich in collagen; however, this scar causes problems in the CNS, preventing complete recovery. CNS recovery appears to be encouraged by early ECM proteins but discouraged by others later, so it is suspected that the interactions of CNS cells with the ECM through different stages influence recovery before forming the chronic glial scar that prevents full healing and return of neuron function across the wound. To test this, three ECMs will be utilized, representing the early clot provisional matrix (FB and FN), the transitional matrix (FB, FN, and collagen), and the fibrous glial scar (FN and collagen). This model will provide a means to study cell-ECM interactions throughout the stages of wound healing in the CNS.