Investigating MroUPO: Insights into Transannular Product Formation and Optimized Cultivation

Abstract

This study aims to expand the scope of MroUPO, a versatile fungal peroxygenase, by identifying whether it possesses the intrinsic ability to form a transannular product and whether using a continuous culture is effective in its cultivation. Various m-CPBA 4-cyclooctenol reactions were conducted. They were compared to a series of MroUPO 4-cyclooctenol enzymatic reactions to see if a transannular product could be produced without the presence of MroUPO. The m-CPBA reactions yielded a transannular product, therefore suggesting that MroUPO does not contain the ability to form a transannular product and can only catalyze its synthesis. To examine the effectiveness of using a continuous culture to cultivate MroUPO, the enzymatic activity and growth of MroUPO cultures were monitored via UV-Vis spectroscopy. Upon introducing a continuous culture, the time it took for MroUPO to reach an enzymatic peak decreased by more than threefold when compared to its natural growth cycle. These results reveal that the use of a continuous culture is effective for MroUPO cultivation.