Browsing by Author "Cai, Amanda"

The rhizosphere is a dynamic chemical environment shaped by secondary metabolites that influence microbial interactions, nutrient availability, and plant health. Among these, ribosomally synthesized, post-translationally modified peptides (RiPPs) represent a largely unexplored class of natural products. DUF692 multinuclear non-heme iron-dependent oxidative enzymes (MNIOs) are an emerging family of tailoring enzymes involved in the biosynthesis of RiPPs. These MNIO-modified RiPPs have shown growing evidence for roles in copper chelation, antivirulence functions, and microbial community dynamics, though their full potential remains underexplored. To address this gap, this study presents a scalable bioinformatic pipeline for identifying MNIO-associated biosynthetic gene clusters (BGCs) across bacterial genomes. Application of this framework uncovered over 1,200 putative MNIO-RiPP BGCs. To probe their functional relevance, we selected a candidate cluster from [Actinomadura] parvosata subsp. kistnae for experimental study. Mass spectrometry analysis revealed a unique mass shift consistent with MNIO-mediated modification, and a putative structure has been proposed. This work expands the known chemical space of MNIO-RiPPs and underscores their potential as bioactive mediators of rhizosphere interactions with translational relevance in environmental and agricultural contexts.